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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
18/06/2018 |
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Data da última atualização: |
18/06/2018 |
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Autoria: |
ANDRADE, C. P.; BARBOSA NETO, J. D.; DRIEMEIER, D. |
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Afiliação: |
Caroline P. Andrade; José D. Barbosa Neto; David Driemeier. |
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Título: |
Identification of single nucleotide polymorphisms in the prion protein gene in Santa Ines and Dorset sheep. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 624-628, abril 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Identificação de polimorfismos de nucleotídeos únicos em ovinos Santa Inês e Dorset através do gene da proteína priônica. |
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Conteúdo: |
Scrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats and results from accumulation of the abnormal isoform of a prion protein in the central nervous system. Resistance or susceptibility to the disease is dependent on several factors, including the strain of infecting agent, the degree of exposure, and the presence of single nucleotide polymorphisms (SNPs) in the prion protein gene. The most important polymorphisms are present in codons 136, 154, and 171. SNPs have also been identified in other codons, such as 118, 127, 141, 142, and 143. The objective of this study was to investigate the genotypic profile of Santa Ines (n=94) and Dorset (n=69) sheep and identify polymorphisms in the prion protein gene using real-time PCR techniques and sequencing. We analyzed SNPs in 10 different codons (127, 136, 138, 140, 141, 142, 143, 154, 171, and 172) in Santa Ines sheep. Classification of the flock into risk groups associated with scrapie revealed that approximately 68% of the Santa Ines herd was considered at moderate risk (group 3), and the most frequent haplotype was ARQ/ARQ (47.8%). For Dorset sheep, 42% of the herd was considered at moderate risk (group 3), 40% at low risk (group 2), and 12% at very low risk (group 1). These findings improve our understanding of the genotype breed and further highlight the importance of genotyping and identification of polymorphisms in Brazilian herds to assess their effects on potential infections upon exposure to the sheep prion. MenosScrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats and results from accumulation of the abnormal isoform of a prion protein in the central nervous system. Resistance or susceptibility to the disease is dependent on several factors, including the strain of infecting agent, the degree of exposure, and the presence of single nucleotide polymorphisms (SNPs) in the prion protein gene. The most important polymorphisms are present in codons 136, 154, and 171. SNPs have also been identified in other codons, such as 118, 127, 141, 142, and 143. The objective of this study was to investigate the genotypic profile of Santa Ines (n=94) and Dorset (n=69) sheep and identify polymorphisms in the prion protein gene using real-time PCR techniques and sequencing. We analyzed SNPs in 10 different codons (127, 136, 138, 140, 141, 142, 143, 154, 171, and 172) in Santa Ines sheep. Classification of the flock into risk groups associated with scrapie revealed that approximately 68% of the Santa Ines herd was considered at moderate risk (group 3), and the most frequent haplotype was ARQ/ARQ (47.8%). For Dorset sheep, 42% of the herd was considered at moderate risk (group 3), 40% at low risk (group 2), and 12% at very low risk (group 1). These findings improve our understanding of the genotype breed and further highlight the importance of genotyping and identification of polymorphisms in Brazilian herds to assess their effects on potential infections upon exposure... Mostrar Tudo |
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Palavras-Chave: |
Genotipagem; Nulceotídeos únicos; Proteína priônica. |
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Thesagro: |
Ovino; Polimorfismo. |
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Thesaurus Nal: |
Genotyping; Sheep; Single nucleotide polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178696/1/Identification-of-single-nucleotide-polymorphisms.pdf
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Marc: |
LEADER 02443naa a2200253 a 4500
001 2092531
005 2018-06-18
008 2018 bl uuuu u00u1 u #d
100 1 $aANDRADE, C. P.
245 $aIdentification of single nucleotide polymorphisms in the prion protein gene in Santa Ines and Dorset sheep.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Identificação de polimorfismos de nucleotídeos únicos em ovinos Santa Inês e Dorset através do gene da proteína priônica.
520 $aScrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats and results from accumulation of the abnormal isoform of a prion protein in the central nervous system. Resistance or susceptibility to the disease is dependent on several factors, including the strain of infecting agent, the degree of exposure, and the presence of single nucleotide polymorphisms (SNPs) in the prion protein gene. The most important polymorphisms are present in codons 136, 154, and 171. SNPs have also been identified in other codons, such as 118, 127, 141, 142, and 143. The objective of this study was to investigate the genotypic profile of Santa Ines (n=94) and Dorset (n=69) sheep and identify polymorphisms in the prion protein gene using real-time PCR techniques and sequencing. We analyzed SNPs in 10 different codons (127, 136, 138, 140, 141, 142, 143, 154, 171, and 172) in Santa Ines sheep. Classification of the flock into risk groups associated with scrapie revealed that approximately 68% of the Santa Ines herd was considered at moderate risk (group 3), and the most frequent haplotype was ARQ/ARQ (47.8%). For Dorset sheep, 42% of the herd was considered at moderate risk (group 3), 40% at low risk (group 2), and 12% at very low risk (group 1). These findings improve our understanding of the genotype breed and further highlight the importance of genotyping and identification of polymorphisms in Brazilian herds to assess their effects on potential infections upon exposure to the sheep prion.
650 $aGenotyping
650 $aSheep
650 $aSingle nucleotide polymorphism
650 $aOvino
650 $aPolimorfismo
653 $aGenotipagem
653 $aNulceotídeos únicos
653 $aProteína priônica
700 1 $aBARBOSA NETO, J. D.
700 1 $aDRIEMEIER, D.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 4, p. 624-628, abril 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
13/12/2012 |
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Data da última atualização: |
25/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
GODOY, A. R.; SALATA, A. C.; CARDOSO, A. I. I.; EVANGELISTA, R. M.; KANO, C.; HIGUTI, A. R. O. |
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Afiliação: |
UNIVERSIDADE FEDERAL DO MATO GROSSO DO SUL; UNESP/FCA; FCAUNESP; UNESP/FCA; CRISTIAINI KANO, CPAA; UNESP/FCA. |
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Título: |
Produção e qualidade pós-colheita de couve-flor em função de doses de potássio em cobertura. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Scientia Agraria Paranaensis, v. 11, n. 2, p. 33-42, 2012. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi avaliar o efeito de doses de potássio em cobertura na produção e qualidade de couve-flor cultivar Teresópolis Gigante. O experimento foi desenvolvido na Fazenda Experimental São Manuel, pertencente à UNESP/FCA, no período de abril a agosto de 2006. |
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Palavras-Chave: |
Adubação potássica; Brassica oleracea v. botrytis; Características físico-químicas. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/72325/1/CFlorPotassio2012.pdf-cristiaini-kano.pdf
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Marc: |
LEADER 00956naa a2200217 a 4500
001 1942587
005 2013-02-25
008 2012 bl uuuu u00u1 u #d
100 1 $aGODOY, A. R.
245 $aProdução e qualidade pós-colheita de couve-flor em função de doses de potássio em cobertura.
260 $c2012
520 $aO objetivo deste trabalho foi avaliar o efeito de doses de potássio em cobertura na produção e qualidade de couve-flor cultivar Teresópolis Gigante. O experimento foi desenvolvido na Fazenda Experimental São Manuel, pertencente à UNESP/FCA, no período de abril a agosto de 2006.
653 $aAdubação potássica
653 $aBrassica oleracea v. botrytis
653 $aCaracterísticas físico-químicas
700 1 $aSALATA, A. C.
700 1 $aCARDOSO, A. I. I.
700 1 $aEVANGELISTA, R. M.
700 1 $aKANO, C.
700 1 $aHIGUTI, A. R. O.
773 $tScientia Agraria Paranaensis$gv. 11, n. 2, p. 33-42, 2012.
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Embrapa Amazônia Ocidental (CPAA) |
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